Where Do Hormones Come From After a Dog Is Spayed?
by Bryson
The ovaries are a female dog's main source of estrogen and progesterone, so once they're removed, she stops coming into heat and those hormone levels fall a lot. Her adrenal glands, two small glands near the kidneys, still make small amounts of sex hormones, so the hormones don't disappear completely.
This drop in estrogen is one reason some spayed females leak urine as they get older, because estrogen helps keep the bladder outlet closed. It's usually treatable, so mention it to your vet if it happens.
If a spayed dog shows heat signs, such as a swollen vulva, bleeding or attracting males, a small piece of ovary may have been left behind, and your vet can check for it.
See spaying dogs.
This is general information, not a diagnosis. Please follow your own vet's advice.